|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS12030 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-bFGFR/FGFR1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human BFGFR C-terminus |
堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格 英文名稱 Anti-bFGFR/FGFR1
中文名稱 堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格
別 名 bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; CEK; FGFBR; FGFR 1; FGF Receptor 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fms like tyrosine kinase 2; Fms related tyrosine kinase 2; Fms related tyrosine kinase 2 Pfeiffer syndrome; H2; H3; H4; H5; HBGFR; Heparin binding growth factor receptor; Hydroxyaryl protein kinase; KAL 2; KAL2; MFR; N SAM; N sam tyrosine kinase; Protein tyrosine kinase; Tyrosylprotein kinase; Basic fibroblast growth factor receptor 1.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 生長(zhǎng)因子和激素
蛋白分子量 predicted molecular weight: 88kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human BFGFR C-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.
Subunit : Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23. Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains). Interacts with FRS2A. Interacts (via C-terminus) with NEDD4 (via WW3 domain). Interacts with KL (By similarity). Interacts with SHB (via SH2 domain) and GRB10. Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3
Subcellular Location : Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.
Tissue Specificity : Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
Post-translational modifications : Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
DISEASE : Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) ; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)是一種多功能的生長(zhǎng)因子,具有有絲分裂和誘導(dǎo)新形成作用,和其受體(FGFR1)堿性成纖維細(xì)胞生長(zhǎng)因子受體結(jié)合之后,在人體各組織中具有廣泛的生物學(xué)活性和生理病理作用.越來越多的研究表明細(xì)胞中bFGF-R1過度表達(dá)對(duì)發(fā)展具有重要意義。
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。
正在熱銷的相關(guān)產(chǎn)品：
PL(Human placetal lactogen) ELISA Kit 人胎盤泌乳素Multi-class antibodies規(guī)格： 48T
Anti-PKB/Akt1 蛋白激酶BMulti-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Heme Oxygenase 1/HO-1 血紅素氧合酶 1/熱休克蛋白32抗體 規(guī)格 0.1ml
MTL(Human Motilin) ELISA Kit 人活性腸肽 96T
PHC2 英文名稱： 早期發(fā)育調(diào)控蛋白2抗體 0.2ml
Cyclin Y 英文名稱： 周期素Y/X抗體 0.2ml
Anti-PKB/Akt1 蛋白激酶BMulti-class antibodies規(guī)格： 0.1ml
Anti-Nadrin/FITC 熒光素標(biāo)記Nadrin抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Phospho-Met (Tyr1234/1235) /FITC 熒光素標(biāo)記磷酸化肝細(xì)胞生長(zhǎng)因子受體(原癌基因)抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh C9orf114 9號(hào)染色體開放閱讀框114抗體 規(guī)格 0.2ml
CD86/B7-2 CD86抗原 0.5mg
hnRNP R 英文名稱： 異質(zhì)性核糖核蛋白R(shí) 0.2ml
Rhesus antibody Rh SCUBE1 新型血小板相關(guān)內(nèi)皮分泌蛋白SCUBE1抗體 規(guī)格 0.2ml
Anti-Phospho-Met (Tyr1234/1235) /FITC 熒光素標(biāo)記磷酸化肝細(xì)胞生長(zhǎng)因子受體(原癌基因)抗體IgGMulti-class antibodies規(guī)格： 0.2ml
RNASE(Human Ribonuclease) ELISA Kit 人核糖核酸酶Multi-class antibodies規(guī)格： 48T
Anti-NGN3 神經(jīng)元素3抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh IL-17A 白介素-17抗體 規(guī)格 0.1ml
apo-A1(Mouse apoprotein) ELISA Kit 小鼠載脂蛋白A1 96T
phospho-PTPN7 (Ser246) 英文名稱： 磷酸化非受體型蛋白酪酸磷酸酶7抗體 0.1ml
CAP2 英文名稱： 環(huán)化酶相關(guān)蛋白CAP-2抗體 0.2ml
Anti-NGN3 神經(jīng)元素3抗體Multi-class antibodies規(guī)格： 0.2ml
ERBB4 Others Rat 大鼠 HER4 / ErbB4 人細(xì)胞裂解液 (陽性對(duì)照)
水貂肺上皮細(xì)胞;Mv.1.Lu(NBL-7)
人平滑肌細(xì)胞完全培養(yǎng)基 100mL
MuM-2C細(xì)胞， 人眼脈絡(luò)細(xì)胞 赭綠青霉 小鼠成纖維細(xì)胞;φ2
ANGPT2 Protein Human 重組人 Angiopoietin-2 / ANG2 蛋白 (Fc 標(biāo)簽)
LLC(小鼠細(xì)胞) 5×106cells/瓶×2 甲型 H2N2 (A/Guiyang/1/1957) 血凝素 (Hemagglutinin / HA) 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)
CM-H003人肺大動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基100mL
NGFR Others Human 人 NGFR/ P75 人細(xì)胞裂解液 (陽性對(duì)照)
I型膠原酶(含10mL酶解緩沖液) 1mL
CAL 27-EGFP(CMV-EGFP慢病毒構(gòu)建穩(wěn)定株)人舌鱗癌細(xì)胞 CAL 27-EGFP (CMV-EGFP leiviral consuct stable sain) of human tongue squamous cell carcinoma DMEM+10% FBS
IL21 Protein Mouse 重組小鼠 IL21 / Ierleukin 21 蛋白
人表皮黑色素細(xì)胞-深色素(HEM)( 5×105 ) MN-h, 小鼠海馬神經(jīng)元 Mouse
堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格 CL-0467WPMY-1(人正常基質(zhì)永生化細(xì)胞)5×106cells/瓶×2
CXCL16 Others Rat 大鼠 CXCL16 / SR-PSOX 人細(xì)胞裂解液 (陽性對(duì)照)
大鼠虹膜色素上皮細(xì)胞完全培養(yǎng)基 100mL
U-373MG人 U-373MG glioma cells DMEM+10% FBS
IL1B Protein Rabbit 重組兔 IL-1 beta / IL1B 蛋白
NCI-H358(人非小細(xì)胞細(xì)胞) 5×106cells/瓶×2 PC-12(腎上腺嗜鉻細(xì)胞瘤細(xì)胞)
技術(shù)外包服務(wù):
★堿性成纖維細(xì)胞生長(zhǎng)因子受體1抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
|
|
|
|
|
