|
| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11928 |
| 應(yīng)用范圍 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-Phospho-Talin (Ser425) |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Talin around the phosphorylation site of Ser425 |
技術(shù)外包服務(wù):
★磷酸化踝蛋白抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
實(shí)驗(yàn)流程：
磷酸化踝蛋白抗體說明書 英文名稱 Anti-Phospho-Talin (Ser425)
中文名稱 磷酸化踝蛋白抗體說明書
別 名 TLN 1; ILWEQ; Talin 1; Talin; TLN; TLN1.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 280kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Talin around the phosphorylation site of Ser425
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化踝蛋白抗體說明書 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.
Function : Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Subunit : Binds with high affinity to VCL and with low affinity to integrins. Interacts with PTK2/FAK1. Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.
Subcellular Location : Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Colocalizes with LAYN at the membrane ruffles.
Similarity : Contains 1 FERM domain.
Contains 1 I/LWEQ domain.
TALIN-踝蛋白。是重要的細(xì)胞骨架肌動(dòng)蛋白結(jié)合蛋白，由兩個(gè)270kDa的亞單位組成的反向平行的同源二聚體，與整合蛋白B尾部的結(jié)合誘導(dǎo)了整合蛋白胞外結(jié)構(gòu)域構(gòu)象的改變,起到了對整合蛋白的激活。
全、新、優(yōu)、品、好四大特點(diǎn)：
磷酸化踝蛋白抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。
正在熱銷的相關(guān)產(chǎn)品：
SOD2 (superoxide-dimutase-2) 超氧化物歧化酶2抗原Multi-class antibodies規(guī)格： 0.5ml
Anti-BNP 腦素/利肽抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Peroxiredoxin 2/PRXII 硫氧還蛋白過氧化物酶Ⅱ/巰基抗氧化蛋白抗體 規(guī)格 0.1ml
Collagen IV 濃縮液 0.1ml 進(jìn)口分裝
TSSK6 英文名稱： 特異絲酸/蘇酸激酶6抗體 0.2ml
Phospho-CDK2 (Thr160) 英文名稱： 磷酸化周期素依賴性激酶2抗體 0.1ml
Anti-BNP 腦素/利肽抗體Multi-class antibodies規(guī)格： 0.1ml
Nanog fragment 干細(xì)胞關(guān)鍵蛋白(多肽)Multi-class antibodies規(guī)格： 0.5mg
Anti-Apaf-1(NT) 凋亡蛋白活性因子-1抗體(N端)Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Phospho-Bad(Ser134) 磷酸化相關(guān)死亡促進(jìn)因子抗體 規(guī)格 0.1ml
山羊抗大鼠 IgG(H+L)/FITC 0.1ml 綠色熒光，Jackson公司分裝
VPS18 英文名稱： 空泡分選蛋白18抗體 0.1ml
Dlx1 英文名稱： 同源轉(zhuǎn)錄因子DLX1抗體 0.2ml
Anti-Apaf-1(NT) 凋亡蛋白活性因子-1抗體(N端)Multi-class antibodies規(guī)格： 0.1ml
Anti-TGF- BetaR 3/FITC 熒光素標(biāo)記轉(zhuǎn)移生長因子–β受體3抗體IgGMulti-class antibodies規(guī)格： 0.2ml
ATF2 (Activating Transcription Factor2) 活化復(fù)制因子2(多肽)Multi-class antibodies規(guī)格： 0.5mg
富含半胱氨酸的酸性分泌蛋白抗體 Anti-SPARC 0.2ml
SRPK1 英文名稱： 絲酸/蘇酸蛋白激酶SRPK1抗體 0.2ml
Ephrin A3 英文名稱： 酪酸蛋白激酶A3受體抗體 0.1ml
Rhesus antibody Rh phospho-MAP4K4(Ser631) 磷酸化原活化蛋白激酶MAP4K4抗體 規(guī)格 0.1ml
ATF2 (Activating Transcription Factor2) 活化復(fù)制因子2(多肽)Multi-class antibodies規(guī)格： 0.5mg
大鼠磷脂酶A2(PL-A2)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
人腎胺酶(RNLS/MAO-C)免疫試劑盒 Human Renalase/ Monoamine Oxidase-C,RNLS/MAO-C ELISA Kit
MousegranzymesA,Gzms-AELISAKit小鼠顆粒酶A(Gzms-A)ELISA試劑盒規(guī)格：96T/48T
人神經(jīng)營養(yǎng)因子3(-3)ELISA試劑盒人神經(jīng)營養(yǎng)因子3(-3)ELISA試劑盒規(guī)格：96T/48T
Humanmaturationpromotingfactor,MPFELISA試劑盒人成熟促進(jìn)因子(MPF)ELISA試劑盒規(guī)格：96T/48T
Humanplaceagrowthfactor，PLGFELISAKit人胎盤生長因子(PLGF)ELISA試劑盒規(guī)格：96T/48T
大鼠補(bǔ)體3a(C3a)ELISA試劑盒 ，英文名： C3a ELISA Kit
Rat superoxide dismase (SOD) ELISA Kit 大鼠超氧化物歧化酶(SOD)ELISA試劑盒
RatcomplemefactorH,CFHELISAKit 大鼠補(bǔ)體因子H(CFH)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforCoxV-IgG(HumanCoxsackievirusIgG)ELISAKit人柯薩奇病毒IgG規(guī)格：48T/96T
細(xì)胞乙醇比色法定量檢測試劑盒20次
RatComplemefragme5a,C5aELISAKit大鼠補(bǔ)體片斷5a(C5a)ELISA試劑盒規(guī)格：96T/48T
磷酸化踝蛋白抗體說明書 大鼠蛋白激酶Cθ(PKCθ)ELISA試劑盒 ，英文名： PKCθ ELISA Kit
N terminal brain naiuretic peptide (-proBNP) ELISA Kit 人N端前腦素(-proBNP)ELISA試劑盒
Ratsolubleierleukin-1receptorⅡ,IL-1sRⅡELISAkit 大鼠白介素1可溶性受體Ⅱ(IL-1sRⅡ)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforCyclin-D1ELISAKit大鼠細(xì)胞周期素D1規(guī)格：48T/96T
細(xì)胞色素P450亞酶CYP2E(AH)活性熒光定量檢測試劑盒20次
Ratferritin,FEELISAKit大鼠鐵蛋白(FE)ELISA試劑盒規(guī)格：96T/48T
|
|
|
|
|
