|
| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11906 |
| 應(yīng)用范圍 | WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600 |
| CAS編號 | |
| 抗體名 | Anti-Tumstatin/COL4A3 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Tumstatin C-terminus |
全、新、優(yōu)、品、好四大特點：
抑素抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。
抑素抗體規(guī)格 英文名稱 Anti-Tumstatin/COL4A3
中文名稱 抑素抗體規(guī)格
別 名 Collagen alpha-3(IV) chain; Alpha 3 type IV collagen; Alpha3 type IV collagen; COL4A 3; COL4A3; Collagen IV alpha 3 polypeptide; Collagen type IV alpha 3 (Goodpasture antigen); Collagen type IV alpha 3; Goodpasture antigen; OTTHUMP00000195044; Tumstatin; Col4a3; CO4A3_HUMAN.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗
研究領(lǐng)域 膠原蛋白
蛋白分子量 predicted molecular weight: 27/159kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Tumstatin C-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
抑素抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.
Function : Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
Subunit : here are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. The alpha 3(IV) chain forms a triple helical protomer with alpha 4(IV) and alpha 5(IV); this triple helical structure dimerizes through NC1-NC1 domain interactions such that the alpha 3(IV), alpha 4(IV) and alpha 5(IV) chains of one protomer connect with the alpha 5(IV), alpha 4(IV) and alpha 3(IV) chains of the opposite promoter, respectively. Interacts with COL4A3BP AND ITGB3. Associates with LAMB2 at the neuromuscular junction and in GBM.
Subcellular Location : Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina(BL).
Tissue Specificity : Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
Post-translational modifications : Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Isoform 2 contains an additional N-linked glycosylation site.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues (By similarity).
Phosphorylated by the Goodpasture antigen-binding protein/COL4A3BP.
DISEASE : Note=Autoantibodies against the NC1 domain of alpha 3(IV) are found in Goodpasture syndrome, an autoimmune disease of lung and kidney.
Defects in COL4A3 are a cause of Alport syndrome autosomal recessive (APSAR) [MIM:203780]. APSAR is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Defects in COL4A3 are a cause of benign familial hematuria (BFH) [MIM:141200]; also known as thin basement membrane nephropathy. BFH is characterized by persistent hematuria, an electron microscopically detectable thin glomerular basement membrane (GBM) and an autosomal dominant mode of inheritance. Renal function remains normal. In children, differentiation between BFH and AS can be difficult, because both disorders are manifested by persistent hematuria and thin GBM at that age.
Defects in COL4A3 are a cause of Alport syndrome autosomal dominant (APSAD) [MIM:104200]. Alport syndrome is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Similarity : Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
Database links : UniProtKB/Swiss-Prot: Q01955.3
抑素（Tumstatin）是來源于基底膜Ⅳ型膠原的生成抑制因子，一種自然產(chǎn)生的大分子多肽蛋白，特異性結(jié)合αvβ3整合素受體選擇性內(nèi)皮細(xì)胞的增殖和遷移，能夠抑制原始外生長出新的，從而、增值，抑素具有很強的生物學(xué)效應(yīng)。
實驗流程：
正在熱銷的相關(guān)產(chǎn)品：
Human Urotensin II 人尾加壓素IIMulti-class antibodies
Anti-DAT1 多巴胺轉(zhuǎn)運蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh NeuN 神經(jīng)元核抗原抗體 規(guī)格 0.1ml
Human Glycated hemoglobin A1c,A1c ELISA Kit 人A1c 96T
TSLP (human) 英文名稱： 胸腺基質(zhì)淋巴細(xì)胞生成素-1抗體(人) 0.2ml
CD69 英文名稱： 活化誘導(dǎo)分子CD69抗體 0.1ml
Anti-DAT1 多巴胺轉(zhuǎn)運蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
Flt3L ELISA Kit 大鼠FMS樣酪氨酸激酶3配體Multi-class antibodies規(guī)格： 48T
Anti-Apo-E/FITC 熒光素標(biāo)記載脂蛋白E抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh FAM50C FAM50C蛋白抗體 規(guī)格 0.2ml
LBP ELISA Kit 大鼠脂多糖結(jié)合蛋白 96T
MPP7 英文名稱： 棕櫚酰化膜蛋白7抗體 0.1ml
ABCG5 英文名稱： 三磷酸腺苷結(jié)合轉(zhuǎn)運蛋白G超家族成員5抗體 0.2ml
Anti-Apo-E/FITC 熒光素標(biāo)記載脂蛋白E抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-TRADD/FITC 熒光素標(biāo)記有死亡區(qū)的壞死因子受體1相關(guān)蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml
PS-1，Presennillin-1(CT) 早老素蛋白-1(S182)Multi-class antibodies規(guī)格： 0.5mg
壞死因子受體相關(guān)因子1抗體 Anti-TRAF1 0.2ml
SorLA/LRP9 英文名稱： 受體相關(guān)蛋白9抗體 0.2ml
ER81 英文名稱： 轉(zhuǎn)錄因子ER81蛋白抗體 0.2ml
Rhesus antibody Rh phospho-ITGB3(Tyr785) 磷酸化整合素β3抗體 規(guī)格 0.1ml
PS-1，Presennillin-1(CT) 早老素蛋白-1(S182)Multi-class antibodies規(guī)格： 0.5mg
大鼠免疫球蛋白A(IgA)ELISA試劑盒 ，英文名： IgA ELISA Kit
人免疫球蛋白M(IgM)ELISA檢測試劑盒HumanImmunoglobulinM,IgMELISAKit 96T/48T
鴨免疫球蛋白E(IgE)免疫試劑盒 Duck Immunoglobulin E,IgE ELISA Kit
CLIAKitforPACAPELISAKit小鼠垂體腺苷酸環(huán)化酶激活肽規(guī)格：48T/96T
水中腸球菌(Eerococci)熒光定量檢測試劑盒20次
ELISAKitCD4人白細(xì)胞分化抗原4規(guī)格：48T/96T
大鼠補體因子H(CFH)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
人免疫球蛋白D(IgD)免疫試劑盒 human Immunoglobulin D,IgD ELISA Kit
MouseVascuoarendothelialcellgrowthfactorreceptor2,VEGFR-2/Flk-1ELISAKit小鼠內(nèi)皮細(xì)胞生長因子受體2(VEGFR-2/Flk-1)ELISA試劑盒規(guī)格：96T/48T
石蠟切片組織CASPASE-4蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測試劑盒10/20次
HumanProstaglandinF2α,PGF2αELISA試劑盒人素F2α(PGF2α)ELISA試劑盒規(guī)格：96T/48T
Humahymosinα1ELISAKit人胸腺肽α1(Thymosinα1)ELISA試劑盒規(guī)格：96T/48T
抑素抗體規(guī)格 大鼠受體相關(guān)蛋白5(LRP5)ELISA試劑盒 ，英文名： LRP5 ELISA Kit
Human FMS like tyrosine kinase 3 ligand (Flt-3L) ELISA Kit 人FMS樣酪氨酸激酶3配體(Flt-3L)ELISA試劑盒
Ratmonocytechemotacticprotein1/monocytechemotacticandactivatingfactor,MCP-1/MCAFELISAkit 大鼠單核細(xì)胞趨化蛋白1(MCP-1/CCL2/MCAF)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforCYP450ELISAKit大鼠細(xì)胞色素P450規(guī)格：48T/96T
細(xì)胞色素P450亞酶CYP2B(EFC)活性熒光定量檢測試劑盒20次
RatFollistatin,FSELISAKit大鼠卵泡抑素(FS)ELISA試劑盒規(guī)格：96T/48T
技術(shù)外包服務(wù):
★抑素抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等
★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
|
|
|
|
|
