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產(chǎn)品展廳
金屬蛋白酶組織抑制因子-2抗體說(shuō)明書(shū)
  • 品牌:上海莼試
  • 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
  • 貨號(hào):CS11816
  • 發(fā)布日期: 2019-01-24
  • 更新日期: 2025-06-09
產(chǎn)品詳請(qǐng)
產(chǎn)地 進(jìn)口、國(guó)產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號(hào) CS11816
應(yīng)用范圍 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
CAS編號(hào)
抗體名 Anti-TIMP-2
克隆性
靶點(diǎn) 詳見(jiàn)說(shuō)明書(shū)
適應(yīng)物種 詳見(jiàn)說(shuō)明書(shū)
形態(tài) 詳見(jiàn)說(shuō)明書(shū)
宿主 詳見(jiàn)說(shuō)明書(shū)
亞型 IgG
標(biāo)識(shí)物 詳見(jiàn)說(shuō)明書(shū)
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human TIMP-2 (125-160aa)

金屬蛋白酶組織抑制因子-2抗體說(shuō)明書(shū) 英文名稱(chēng)  Anti-TIMP-2

中文名稱(chēng)  金屬蛋白酶組織抑制因子-2抗體說(shuō)明書(shū) 

     Metalloproteinase inhibitor 2 precursor; Tissue inhibitor of metalloproteinases 2; Collagenase inhibitor; CSC 21K; CSC21K; TIMP2; TIMP 2; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP2_HUMAN; Metalloproteinase inhibitor 2; CSC-21K; TIMP-2.

產(chǎn)品屬性:

      1mg/1ml

規(guī)   0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     心 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 生長(zhǎng)因子和激素 合成與降解

蛋白分子量  predicted molecular weight: 24kDa 

       Lyophilized or Liquid

  KLH conjugated synthetic peptide derived from human TIMP-2 (125-160aa)

      IgG

純化方法   affinity purified by Protein A

儲(chǔ)    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

金屬蛋白酶組織抑制因子-2抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008].

Function : Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Subunit : Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity.

Subcellular Location : Secreted.

Post-translational modifications : The activity of TIMP2 is dependent on the presence of disulfide bonds.

Similarity : Belongs to the protease inhibitor I35 (TIMP) family.

Contains 1 NTR domain.

Database links :

UniProtKB/Swiss-Prot: P16035.2

Entrez Gene: 282093 Cow

Entrez Gene: 100135629 Guinea pig

Entrez Gene: 7077 Human

Entrez Gene: 21858 Mouse

Entrez Gene: 100008689 Rabbit

Entrez Gene: 29543 Rat

Omim: 188825 Human

SwissProt: P16368 Cow

合成與降解(Synthesis and Degradation)

TIMP-2與TIMP-1一樣是基質(zhì)金屬蛋白酶的抑制酶,其功能與MMP相反,能細(xì)胞的浸潤(rùn)和轉(zhuǎn)移,主要用于各種如等的研究。TIMP-2又稱(chēng)為CSC-21K,是一種21-24kDa的糖蛋白,在多種組織細(xì)胞中表達(dá),它可以和MMPs家族成員以共價(jià)鍵方式形成復(fù)合物,從而發(fā)揮其。在正常生理?xiàng)l件下,MMPs和TIMPs之間的平衡狀態(tài)是維持細(xì)胞外基質(zhì)正常狀態(tài)所必需的,一旦它們之間的比例失調(diào),就會(huì)造成一系列的病理狀態(tài)。

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