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| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11750 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-TRPV3 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human TRPV3 |
產(chǎn)品訂購信息：
英文名稱 Anti-TRPV3
中文名稱 辣椒素受體3抗體規(guī)格
別 名 1110036I10Rik; MGC124324; MGC124325; Transient receptor potential cation channel subfamily V member 3; TrpV3; Trpv3 heat sensitive channel; TRPV3_HUMAN; Vanilloid receptor 3; Vanilloid receptor like 3; Vanilloid receptor-like 3; vanilloid receptor-related osmotically activated channel protein; VRL 3; VRL-3; VRL3.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細胞生物 神經(jīng)生物學(xué) 通道蛋白 細胞膜受體
蛋白分子量 predicted molecular weight: 91kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TRPV3
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
辣椒素受體3抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Transient receptor potential (TRP) proteins are cation-sensitive channels that modulate a myriad of cellular functions, including temperature sensation and vasoregulation Transcribed from a gene adjacent to VR-1, the thermal-sensitive, capsaicin-insensitive TRPV3 is expressed at warm temperatures; expression increases in response to noxious temperatures. Human TRPV3 is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. In addition, TRPV3 is co-expressed in dosal root ganglion neurons with VR-1. TRPV3 associates with VR-1 and may modulate VR-1 activity. The 729 amino acid TRPV5 (ECAC1) protein comprises six transmembrane domains, multiple potential phosphorylation sites, an N-linked glycosylation site and three ankyrin repeat regions. It is abundantly expressed in kidney, jejunum and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. TRPV5 controls the rate-limiting step of vitamin D3-regulated Ca2+ reabsorption in kidney and intestine; the 5’-flanking region of TRPV5 contains four putative vitamin D3-responsive elements.
Function : Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
Subunit : May form a heteromeric channel with TRPV1. Interacts with TRPV1.
Subcellular Location : Membrane; Multi-pass membrane protein.
Tissue Specificity : Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
DISEASE : Defects in TRPV3 are the cause of Olmsted syndrome (OLMS) [MIM:614594]. A rare congenital disorder characterized by bilateral mutilating palmoplantar keratoderma and periorificial keratotic plaques with severe itching at all lesions. Diffuse alopecia, constriction of digits, and onychodystrophy have also been reported. Infections and squamous cell carcinomas can arise on the keratotic areas. The digital constriction may progress to autoamputation of fingers and toes.
Similarity : Belongs to the transient receptor (TC 1.A.4) family.
TrpV subfamily. TRPV3 sub-subfamily.
Contains 3 ANK repeats.
Database links : UniProtKB/Swiss-Prot: Q8NET8.2
Anti-phosphor-PAK1+PAK2+PAK3 (pSer141)/FITC 熒光素標記抗磷酸化p21激活激酶1/2/3抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-pig IgG/Gold 金標記兔抗豬IgG(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml
FosB抗體 Anti-FOSB 0.2ml
Goat Anti-rabbit IgG/Alexa Fluor 488 Alexa Fluor 488標記的羊抗兔IgG 0.1ml
FNDC3A 英文名稱： Ⅲ型纖維連接蛋白域蛋白3A抗體 0.2ml
Rhesus antibody Rh phospho-STAT5a(Ser780) 磷酸化信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子5a抗體 規(guī)格 0.1ml
Rabbit Anti-pig IgG/Gold 金標記兔抗豬IgG(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml
IL-1 Alpha (Interleukin-1 Alpha ) 白介素1α抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-F-Actin 纖維狀肌動蛋白抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-CDK5(Tyr15) 磷酸化周期素依賴性激酶5抗體 規(guī)格 0.1ml
品名 規(guī)格 備注
WDR61 英文名稱： WD重復(fù)膜蛋白61抗體 0.2ml
DOCK2 英文名稱： 細胞質(zhì)分裂付出蛋白2抗體 0.2ml
Anti-F-Actin 纖維狀肌動蛋白抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-AFP/FITC 熒光標記鼠抗人甲胎蛋白單克隆抗體IgGMulti-class antibodies規(guī)格： 0.2ml
DP-I/DSP(desmoplakin I ) 橋粒斑蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg
載脂蛋白H抗體 Anti-ApoH 0.1ml
ZSWIM3 英文名稱： ZSWIM3蛋白抗體 0.2ml
DERP6 英文名稱： 表皮源性蛋白6抗體 0.2ml
Rhesus antibody Rh phospho-EPH A3+A4+A5 ( Tyr779) 磷酸化內(nèi)皮細胞受體蛋白酪氨酸激酶A3+A4+A5抗體 規(guī)格 0.1ml
DP-I/DSP(desmoplakin I ) 橋粒斑蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg
人阿米巴(Amebiasis)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
兔子窖蛋白Caveolin-1(CAV1)免疫試劑盒 Rabbit Caveolin-1,CAV1 ELISA kit
神經(jīng)母細胞瘤抗體(NB-Ab)ELISA試劑盒 ，英文名： NB-Ab ELISA Kit
Porcineierleukin17,IL-17ELISA試劑盒豬白介素17(IL-17)ELISA試劑盒規(guī)格：96T/48T
Humanadvancedglycationendproducts,AGEsELISA試劑盒人晚期糖基化終末產(chǎn)物(AGEs)ELISA試劑盒規(guī)格：96T/48T
HumanAi-ypsin,ATELISAKit人抗胰蛋白酶(AT)ELISA試劑盒規(guī)格：96T/48T
大鼠水通道蛋白4(AQP-4)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
人同型半胱氨酸(Hcy)免疫試劑盒 Human Homocysteic acid,Hcy ELISA Kit
β-葡萄糖苷酶(β-GC)測試盒 可見分光光度法 50管/24樣
RatsolubleP-selectin,sP-selectinELISA試劑盒大鼠可溶性P選擇素(sP-selectin)ELISA試劑盒規(guī)格：96T/48T
Humankaliureticpeptide,KPELISA試劑盒人肽(KP)ELISA試劑盒規(guī)格：96T/48T
HumanNeonatalThyroxine,NN-T4ELISAKit人新生甲狀腺素(NN-T4)ELISA試劑盒規(guī)格：96T/48T
辣椒素受體3抗體規(guī)格 大鼠維生素D2(VD2)ELISA試劑盒 ，英文名： VD2 ELISA Kit
人葡萄糖依賴性釋放多肽(GIP)ELISA檢測試劑盒HumanGlucosedependeinsulieleasingpolypeptide,GIPELISAKit 96T/48T
倉鼠ELISA試劑盒
CLIAKitforRBP(Humaetinolbindingprotein)ELISAKit人視黃醇結(jié)合蛋白規(guī)格：48T/96T
石蠟切片糖原高酸希爾(PAS)法染色試劑盒50次
ELISAKitCasp-3人胱天蛋白酶3規(guī)格：48T/96T
抗體的生物素化標記實驗要點：
1. 辣椒素受體3抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。
抗體的鑒定：
1）辣椒素受體3抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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