|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS11312 |
| 應(yīng)用范圍 | WB= ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-phospho-Separase (Ser1073) |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
| 宿主 | 詳見(jiàn)說(shuō)明書(shū) |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073 |
全、新、優(yōu)、品、好四大特點(diǎn)：
磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū) 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。
磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū) 英文名稱 Anti-phospho-Separase (Ser1073)
中文名稱 磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū)
別 名 Caspase like protein ESPL1; ESP 1; ESP1; ESP-1; ESPL 1; ESPL1; ESPL-1; Extra spindle poles like 1; Extra spindle poles like 1 protein; Separin; Similar to fission yeast cut1and gene; SSE; Separase; ESPL1_HUMAN.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)運(yùn)蛋白
蛋白分子量 predicted molecular weight: 198kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用 WB= ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers.
Function : Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Subunit : Interacts with PTTG1. Interacts with RAD21.
Subcellular Location : Cytoplasmic and Nuclear
Post-translational modifications : Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Similarity : Belongs to the peptidase C50 family.
實(shí)驗(yàn)流程：
正在熱銷的相關(guān)產(chǎn)品：
Anti-TNK1/FITC 熒光素標(biāo)記非受體型酪氨酸蛋白激酶Tnk1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
CCL4/MIP-1 Beta(Macrophage Inflammatory Protein 1 beta) 巨噬細(xì)胞炎性蛋白1β抗原Multi-class antibodies規(guī)格： 0.5mg
酶2抗體 Anti-CA Ⅱ 0.1ml
SEMA4B 英文名稱： 跨膜蛋白SEMA4B抗體 0.2ml
E2F1 英文名稱： 轉(zhuǎn)錄因子E2F-1抗體 0.1ml
Rhesus antibody Rh phospho-HSF1(Ser307) 磷酸化熱休克因子1抗體 規(guī)格 0.1ml
CCL4/MIP-1 Beta(Macrophage Inflammatory Protein 1 beta) 巨噬細(xì)胞炎性蛋白1β抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-ProADM (Adrenomedullin)-N20 peptide /FITC 熒光素標(biāo)記腎上腺髓質(zhì)素前提N端20肽抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-human IgA Whole serum 兔抗人IgA抗血清Multi-class antibodies規(guī)格： 1ml
白細(xì)胞介素-16抗體 anti-IL-16/LCF 0.2ml
Rabbit anti-mouse IgG-Fc fragment whole serum 兔抗小鼠IgG-Fc段抗血清 1ml
Factor I light chain 英文名稱： 補(bǔ)體因子I輕鏈抗體 0.2ml
Rhesus antibody Rh phospho-PTPN6(Ser591) 磷酸化蛋白酪氨酸磷酸酶1C抗體 規(guī)格 0.1ml
Rabbit Anti-human IgA Whole serum 兔抗人IgA抗血清Multi-class antibodies規(guī)格： 1ml
IRAK 2(IL-1 Receptor-associated Kinase 2) 白介素-1受體相關(guān)激酶2抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-ADORA3 腺苷受體A3抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-CD32B(Tyr291) 磷酸化CD32B抗體 規(guī)格 0.1ml
單組分DAB染色試劑盒 100ml/500ml 黃色,用于IHC和WB
VG5Q 英文名稱： 生長(zhǎng)因子VG5Q抗體 0.2ml
DPP3 英文名稱： 二肽基肽酶3抗體 0.2ml
Anti-ADORA3 腺苷受體A3抗體Multi-class antibodies規(guī)格： 0.2ml
IFNA8 Protein Human 重組人 Ierferon alpha-B / IFNA8 蛋白
大鼠滑膜細(xì)胞完全培養(yǎng)基 100mL
ADIPOQ Others Human 人 Adiponectin / Acrp30 / ADIPOQ 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
RSC96大鼠雪旺細(xì)胞 Schwann cells in RSC96 rats DMEM+10%FBS
肝實(shí)質(zhì)細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
HuTu-80(人十二脂腸腺癌) 5×106cells/瓶×2 宋內(nèi)氏志賀氏菌(Sh.sonnei)
EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞(彝族);KM934
CD302 Others Mouse 小鼠 CD302 / CLEC13A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
H4 人神經(jīng)膠質(zhì)細(xì)胞瘤
RKO-E6人轉(zhuǎn)基因細(xì)胞 Human colon cancer RKO-E6 ansgenic cells MEM培養(yǎng)基(GIBCO)+10%FBS
TNFSF13B Protein Human 重組人 BLyS / TNFSF13B / BAFF 蛋白
人臍帶間葉干細(xì)胞(臍帶)(HUMSC) (5×105) AGS人胃腺癌細(xì)胞 Human
磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū) IFNA8 Protein Human 重組人 Ierferon alpha-B / IFNA8 蛋白
大鼠滑膜細(xì)胞完全培養(yǎng)基 100mL
ADIPOQ Others Human 人 Adiponectin / Acrp30 / ADIPOQ 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
RSC96大鼠雪旺細(xì)胞 Schwann cells in RSC96 rats DMEM+10%FBS
肝實(shí)質(zhì)細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
HuTu-80(人十二脂腸腺癌) 5×106cells/瓶×2 宋內(nèi)氏志賀氏菌(Sh.sonnei)
技術(shù)外包服務(wù):
★磷酸化外紡錘體極樣蛋白1抗體說(shuō)明書(shū) 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
|
|
|
|
|
